PG entrance exam CUET PG 2025 Mock Test Life Sciences Methods in Biology Molecular Biology Techniques
A student was asked to clone a DNA fragment, flanked by BamHI (GGATCC) sites into a vector, cut with Bglll (AGATCT). The cloning was successful, as these two restriction enzymes are known to create compatible sites. Based on this, what will be the restriction site configurations of these two enzymes (indicates the point of enzymatic cleavage).
1
G/GATCC and AGATC/T
2
G/GATCC and A/GATCT
3
GGATC/C and A/GATCT
4
GGATC/C and AGA/TCT