Fill in the blanks with appropriate prepositions from the options given below:

Rennet casein is produced ________ skin milk by coagulation _______ rennet. Soluble calcium ions are required _______ this reaction. Sufficient quantities ________ calcium is already present in milk.

1
from, with, for, of
2
from, with, of, for
3
from, in, with, for
4
from, of, for, with

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