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Given below is a DNA sequence:
5' - ATGCGATGACGATTGACGATGACGATAGAC - 3'
In the absence of any other affecting parameters such as length, Tm, GC-content, which one of the following combinations of PCR primer sequences would be able to amplify the above fragment?
1
5'- TACGCTAC - 3' and 5' - GTCTATCG - 3'
2
5'- CGATAGAC - 3' and 5' – ATGCGATG - 3'
3
5'- ATGCGATG - 3' and 5'-CAGATAGC - 3'
4
5'- CATCGCAT - 3' and 5'-CGATAGAC - 3'