Plasmid pBR322 has a Pst l restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β -galactosidase production and the recombinant plasmid is inserted in an E.coli strain
1
It will be able to produce a novel protein with dual ability
2
It will not be able to confer ampicillin resistance to the host cell
3
The transformed cells will have the ability to resist ampicillin as well as produce β -galactoside
4
It will lead to lysis of host cell