DNA gyrase (a type of Topoisomerase II) activity was measured in terms of changes in the supercoiling of DNA in the presence of Ciprofloxacin (an inhibitor of DNA gyrase) or ICRF-193 (an inhibitor of Topoisomerase II). Which one of the following is the correct expected outcome?

1
In the presence of Ciprofloxacin, DNA gyrase will introduce negative supercoiling in the DNA.
2
In the presence of ICRF-193, DNA gyrase will introduce positive supercoiling in the DNA.
3
In the presence of Ciprofloxacin, DNA gyrase will be unable to introduce negative supercoiling in the DNA.
4
In the presence of ICRF-193, DNA gyrase will be able to introduce negative supercoiling in the DNA.
5
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