The B form double stranded DNA was invaded by a complementary RNA sequence to form an R-­loop structure. During this process, 

1
sugar puckering on the DNA strand that pairs with RNA will remain unchanged. 
2
sugar puckering on the DNA strand that pairs with RNA will change.
3
sugar puckering on the DNA strand that pairs with RNA will remain unchanged but the number of base pairs per turn in the RNA‐DNA hybrid will increase. 
4
sugar puckering on the DNA strand that pairs with RNA will change but the number of base pairs per turn in the RNA‐DNA hybrid with remain unchanged.
5
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