In the transformation of oxyhaemoglobin to deoxyhaemoglobin

1
Fe3+ in the low spin changes to Fe2+ in the high spin state
2
Fe2+ in the low spin remains Fe2+ in the low spin state
3
Fe2+ in the high spin changes to Fe2+ in the low spin state
4
Fe2+ in the high spin remains Fe2+ in the high spin state
5
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