The DNA sequence shown below is to be amplified by PCR:
5' GCTAAGATCTGAATTTTCC ...... TTGGGCAATAATGTAGCGC3'
3' CGATTCTAGACTTAAAAGG ...... AACCCGTTATTACATCGCG5'
Which one of the following pair of primers can be used for this amplification?
1
5' GGAAATTCAGATCTTAGT3' and 5' TGGGCAATAATGTAGCGC3'
2
5' GCTAAGATCTGAATTTTC3' and 5' GCGCTACATTATTGCCCA3'
3
5' CGGAAATTCAGATCTTAG3' and 5' GCGCTACATTATTGCCCA3'
4
5' GCTAAGATCTGAATTTTC3' and 5' TGGGCAATAATGTAGCGC3'
5
Question Not Attempted