In the Yeast Two-Hybrid system for studying protein-protein interactions, which component is necessary for the reporter gene activation only when two specific proteins interact within the yeast cell?

1
DNA-Binding Domain (DBD) alone.
2
Activation Domain (AD) alone.
3
Both DNA-Binding Domain (DBD) and Activation Domain (AD) fused to the interacting proteins.
4
Ligase enzyme that joins fragmented DNA strands.
5
Question Not Attempted

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