Which of the following statements is correct about Edman degradation?

1
The peptide bonds are sequentially hydrolyzed from the N-terminus, and the released amino acid is derivatized and analyzed by HPLC.
2
The derivation of N-terminal amino acids is is identified by its fluorescent properties 
3
The reaction cannot be carried out on the same sample of the peptide for sequencing
4
The reaction can be used in reverse for the synthesis of peptides
5
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