Results of immunoprecipitation (IP) of HA-Yfg are shown below.
Given below are options of controls that could be used to confirm that F1β actually associates with HA-Yfg.
A. Include a lane where α-HA is not added but Protein A-Sepharose is added
B. Include a lane where neither α-HA nor Protein A-Sepharose are added
C. Include a lane where α-HA is added but Protein A-Sepharose is not added
D. Include a lane where α-Myc is added instead of α-HA before addition of Protein A-Sepharose.
Which one of the following options represent(s) the most appropriate control(s)?
1
A only
2
A and B
3
A and D
4
C and D