A conjugation experiment is carried out between F+ his+ leu+ thr+ pro+ bacteria and F- his- leu- thr- pro- bacteria for a period of 25 minutes. At this time the mating is stopped, and the genotypes of the recipient F- bacteria are determined. The results are shown below:

Genotype

Number of colonies

His+

0

Leu+

13

Thr+

26

Pro+

6


What is the probable order of these genes on the bacterial chromosome?

1
thr, leu, pro, his
2
pro, leu, thr and the position of his cannot be determined
3
thr, leu, pro, and the position of his cannot be determined.
4
his, pro, leu, thr

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