The theoretical resolution limit of the fluorescence microscope is about 200 nm . Super-resolution microscopy has been developed to address this limitation. Given below are super-resolution microscopy methods in column X and their principle in column Y.
| Super-resolution microscopy (Column X) | Principle (Column Y) | ||
| A. | Structured illumination microscopy (SIM) | (I) | focused excitation laser point is surrounded by donut-shaped depletion beam |
| B. | Stimulated emission depletion (STED) microscopy | (lI) | the specimen is illuminated with a pattern of light and dark stripes to generate Moire fringes |
| C. | Photoactivated localization microscopy (PALM) | (llI) | utilizes variant of GFP that is activated by a wavelength different from its excitation wavelength |
Which one of the following options represents the correct match between column X and column Y?
1
A - (i), B - (ii), C - (iii)
2
A - (ii), B - (i), C - (iii)
3
A - (iii), B - (ii), C - (i)
4
A - (ii), B - (iii), C - (i)