Normal human fibroblasts, cancer cells (that originated in stem cells) and fibroblasts transduced with hTERT (hTERT cells) were passaged for 35 generations. Southern blot analysis was performed using DNA from above cells using radio-labelled probes for telomeric sequences. Which one of the following band patterns would be observed in the autoradiogram?
1
7-9 kb bands in fibroblasts, 18-20 kb bands in cancer and hTERT cells.
2
18-20 kb bands in fibroblasts, 7-9 kb bands in cancer and hTERT cells.
3
7-9 kb bands in fibroblasts and hTERT cells, 18-20 kb bands in cancer cells.
4
18-20 kb bands in fibroblasts and hTERT cells, 7-9 kb in cancer cells.