A researcher overexpresses the full-length genomic sequence of rice GAPDH gene under a CaMV35S promoter in transgenic rice. Which one of the following options can be used to confirm transgenic lines using PCR?
1
Using exon-specific primers of the GAPDH gene.
2
Using intron-specific primers of the GAPDH gene.
3
Amplification of the promoter region of the GAPDH gene.
4
Using CaMV35S promoter-specific forward and GAPDH-specific reverse primer.