Which one of the following statements related to genetic transformation of plants is correct? 

1
Negative selection markers need to be expressed only under strong constitutive promoters for development of transgenic plants 
2
A transgenic plant containing two linked copies of the transgene in heterozygous condition would segregate in a 3 ∶ 1(transgenic : non‐ transgenic) ratio for the transgenic phenotype on self pollination. 
3
Agrobacterium –mediated transfer of T‐DNA into a host plant does not require host plant proteins. 
4
In a binary vector system of Agrobacterium tumefaciens, the oncogenes are located on the Helper plasmid. 

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