In a study of enzyme kinetics, the presence of a non-competitive inhibitor is found to affect the reaction kinetics of an enzyme-catalyzed reaction. What is the primary effect of this type of inhibition?

1
 It decreases both Km and Vmax.
2
It decreases Vmax without affecting Km.
3
It increases Km without affecting Vmax.
4
 It has no effect on either Km or Vmax.

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