Which of the following statement regarding the oxygen dissociation curve is true?

1
The quaternery structure of haemoglobin does not determines its affinity to O2.
2
In deoxyhaemoglobin, the globin units are loosely bound in a T- configuration.
3
The interactions between globin subunits are altered O2 binds with deoxyhemoglobin
4
The affinity to O2 in T-configuration of haemoglobin is increased. 

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