Which one of the following statements is TRUE?

1
Nick translation cannot be used for radio-labeling DNA fragments for use in Southern hybridization
2
RACE can be used to obtain sequence information of the ends of a cDNA
3
In a 2-D gel electrophoresis for separating proteins, analytes are first separated on the basis of their size and then on the basis of their pl.
4
AFLP markers are typically used to screen heterozygotes from homozygotes

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