Teaching MPPSC Assistant Professor Mock Test Series 2025 Life Sciences Methods in Biology Biophysical Method
A protein sample isolated from natural source was electrophoresed on a non reducing SDS PAGE. It migrated as a band corresponding to 60 kDa. When the same protein was electrophoresed on a native PAGE its migration corresponded to a 120 kDa marker protein. What is a reasonable inference?
1
The protein is a dimer of 60 kDa subunits that are not linked with disulfides
2
The protein has two 60 kDa subunits that are linked with disulfides.
3
No inference concerning protein size can be drawn since proteins don't run according to their masses in native PAGE.
4
The protein is unusually rich in aspartic and glutamic acid