Which one of the following is active transport?

1
It is the movement of a substance against a diffusion gradient with the use of energy from respiration.
2
It is the movement of a substance against a diffusion gradient without the use of energy.
3
It is the movement of a substance against a diffusion gradient with the use of energy from photosynthesis.
4
It is the movement of a substance along a diffusion gradient with the use of energy from respiration.

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