A gene is inserted into the PvuII site of the cloning vector (given below) and transformed into E. coli cells. Which one of the following statements is then true?
1
Recombinants can be selected by plating on ampicillin containing medium.
2
Recombinants can be selected by plating on tetracycline containing medium.
3
Recombinants cannot be selected by plating either on ampicillin or tetracycline containing medium.
4
Recombinants can be selected by plating simultaneously on ampicillin and tetracycline containing medium.