Establishment of mammalian cell culture involves the following steps: A. Culture of primary cells B. Subculture of primary cells C. Separation of cells from tissue by mechanical or enzymatic method D. Culture of secondary cells E. Subculture of secondary cells Choose the correct answer from the options given below:
1
C, A, B, D, E
2
A, C, E, B, D
3
A, D, C, B, E
4
A, D, B, E, C