The following is the list of steps involved in cloning of a gene.
Which option represents the correct sequence of the process?
(A). Transformation with blue and white selection.
(B). RFLP analysis.
(C). Isolation of plasmid DNA.
(D). Ligation of gene, into TA-cloning vector.
(E). Amplification of gene by PCR, using Taq DNA polymerase.
Choose the correct answer from the options given below:
1
(E), (D), (C), (B), (A).
2
(E), (B), (D), (C), (A).
3
(C), (E), (D), (B), (A).
4
(C), (B), (D), (A), (E).