In an experiment, two fly populations are separately maintained, for many generations. Population A, contains closely related individuals, whereas, population B contains a set of unrelated individuals. Over many generations, it was observed that the fitness of individuals, in population A, is lower than the fitness of those in Population B, because -

1
Close relatives will not mate with one another.
2
Close relatives compete for common resources more than unrelated individuals. 
3
The offspring of close relatives harbour, more deleterious recessive mutations.
4
Unrelated individuals are likely to contain new high-fitness genes.

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