Sub-cellular fractionation-based assays have been used to identify various organelles in mammalian cells. In order to characterize such organelles in a living mammalian cell, which of the following microscopy-based method would be the most accurate?

1
use of fluorescent probes specific for organelles.
2
use of organelle specific fluorescent probes followed by microinjection of fluorescent antibodies against organelle- specific protein.
3
use of fluorescent probes in permeabilized cells
4
use of organelle specific fluorescent probes followed by cryo-electron microscopy

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