When collecting a sputum specimen for culture and sensitivity testing, which of the following is the most critical instruction to share with the patient?

1
Collect the specimen after eating to ensure a more substantial sample.
2
Rinse the mouth with an antiseptic mouthwash immediately before collection.
3
Provide a specimen that comes from deep coughing.
4
Drink water before collection to increase the volume of sputum.

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