Which if the following criterion is not related to Koch's postulates or Koch's phenomena or Koch's laws?

1
The bacterium should be constantly associated with the lesion of the disease.
2
It should be possible to isolate the bacterium in pure culture from the lesions.
3
Inoculations of such pure culture into suitable laboratory animals should reproduce the lesions of the disease.
4
It would not be possible to reisolate the bacterium in pure culture from the lesions produced in the experimental animals.

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