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Researchers at the Indian Institute of Science (IISc) have developed a low-cost and effective method to detect adulterants in milk, by analysing deposition patterns after evaporation. In a study published in ACS Omega, the team used the method to test for the presence of urea and water – the most common adulterants – but they suggest that such a technique can also be extended to other adulterants. Adulteration of milk is a pressing concern in developing countries like India, where a majority of supplied milk fails to comply with the standards set by the Food Safety and Standards Authority of India. Water is frequently added to increase the volume of milk, along with urea, which makes the watered-down version whiter and foamier – this can potentially endanger the normal functioning of the liver, heart, and kidneys. In this technique, the researchers looked at evaporative deposition patterns – those that emerge when a liquid mixture like milk completely evaporates, causing volatile components to dissipate, and solids or non-volatile components to arrange themselves in distinctive patterns. Milk with and without water or urea showed very different evaporative patterns. In unadulterated milk, the evaporative pattern consisted of a central, irregular blob-like pattern. Water was found to cause distortion or complete loss of this distinctive pattern, depending on how much is added. Urea also completely erases the central pattern; being a non-volatile component, it does not evaporate but instead crystallises, starting at the interior of the milk drop and extending along the periphery. Current techniques such as using a lactometer and looking for changes in the freezing point of milk can be used to detect the presence of water, but they have certain limitations. For instance, the freezing point technique can detect water only up to 3.5% of the total milk concentration. In addition, although biosensors with high sensitivity are available to test for urea, they are expensive, and their accuracy tends to decrease with time. The IISc team, on the other hand, was able to detect water concentrations as high as 30% and urea concentrations in diluted milk as low as 0.4% using this type of pattern analysis. Therefore, the technique could be a convenient replacement when access to such equipment is not available.