How does the Enzyme Linked Immuno-sorbent Assay (ELISA) work in the detection of infections?

1
 It amplifies the DNA of pathogens to detect them.
2
It uses radioactive probes to hybridize with pathogen DNA
3
It detects the presence of antigens or antibodies in a sample through antigen-antibody interactions.
4
It identifies mutations in genes by comparing sequences.

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