Following statements were given regarding factors influencing variation in expression levels of transgene in transgenic plants:

A. Difference in restriction enzyme sites within the T-DNA.

B. Difference in copy number of the transgene.

C. Variations in site of integration of the T-DNA within the plant genome.

D. Presence of multiple promoters within the T-DNA region.

Which one of the following options represents a combination of statements that would NOT lead to variations in transgene expression levels in transgenic plants generated using the same T-DNA/binary vector?