Which one of the statements given below regarding the generation of monoclonal antibodies is INCORRECT?
1
Monoclonal antibodies are the product of a single stimulated B‐lymphocyte.
2
To generate large quantity of monoclonal antibodies, a normal stimulated antibody producing B cell is fused with a long lived B cell tumor.
3
The hybridoma generated for antibody production is selected on HAT medium.
4
For HAT selection of hybridoma, the antibody-producing B‐cells are pre‐treated with 8‐azaguanine to block the salvage pathway of DNA synthesis.