Teaching DSSSB PGT Mock Test 2025 Chemical Sciences Characterisation of Inorganic Compounds UV-Vis Spectroscopy
A protein has 3 tyrosine residues and 'n' tryptophan residues both of which are the only amino acids absorbing at 280nm. If the absorbance of the protein having a concentration of 10μM (in a cuvette of path length 2 cm) is 0.59, the number of tryptophan residues in the protein must be
[Given: ε280 (Tyrosine) = 1500 M-1cm-1
ε280 (Tryptophan) = 5000 M-1 cm-1]
1
11
2
5
3
2
4
7