During the process of translation initiation in prokaryotes, the assembly of the initiation complex on the mRNA involves several initiation factors (IFs). What is the correct order of involvement of these factors in forming the initiation complex?
1
IF3 binds to the small ribosomal subunit, preventing its association with the large subunit; IF1 and IF2 with GTP then facilitate the binding of fMet-tRNAfMet to the start codon.
2
IF2 with GTP binds to the small ribosomal subunit first, followed by IF1 facilitating the binding of fMet-tRNAfMet, and IF3 preventing premature association with the large subunit.
3
IF1 binds to the small ribosomal subunit, enhancing the binding affinity of IF2 and fMet-tRNAfMet; IF3 joins last to assist in locating the start codon.
4
IF3 and fMet-tRNAfMet bind together to the mRNA first, followed by simultaneous recruitment of IF1 and IF2 with GTP to the small ribosomal subunit.
5
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