tRNA genes of a Gram positive bacteria were sequenced. While some of the genes possessed sequence corresponding to the –CCA end of the tRNA, others did not. Interestingly, even the genes that lacked the sequence corresponding to the –CCA end of the tRNA were found to code for a functional tRNA. This is because
1
the –CCA end in the tRNA is not essential for its function.
2
the genes that do not possess sequence corresponding to the –CCA ends of the tRNA are repaired prior to their transcription.
3
the –CCA end is added to the tRNA during their maturation.
4
the primary transcripts of the genes lacking sequence corresponding to the – CCA end are subjected to g-RNA mediated editing prior to their maturation
5
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