Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are 5.6 and 6.8. Which one of the following techniques can be used to separate them?
1
Denaturing polyacrylamide gel electrophoresis
2
Size-exclusion chromatography
3
Ion-exchange chromatography
4
Nickel affinity chromatography
5
Question Not Attempted