During transgenesis, the location of the genes and their number integrated into the genome of the transgenic animal are random. It is often necessary to determine the copy number of genes and their tissue-specific transcription. The following are the possible methods used for the determination.

A. Polymerase Chain Reaction(PCR)

B. Southern blot hybridization

C. Reverse Transcriptase PCR

D. Western blot

Choose the correct set of combinations.