Agrobacterium tumefaciens, a soil bacterium, is well known for its ability to transfer T-DNA into plant cells, a process central to plant genetic engineering. The vir genes play a crucial role in this process. Select all correct statements regarding the vir gene-encoded proteins and their specific functions in T-DNA transfer:

A) VirA functions as a sensor kinase in the bacterial cell membrane that detects phenolic compounds exuded by wounded plant cells, initiating the T-DNA transfer process.
B) VirG acts as a response regulator that directly interacts with plant auxins to facilitate T-DNA integration into the plant genome.
C) VirD2 is an endonuclease that cleaves the T-DNA at the border sequences and remains covalently attached to the 5' end of the T-DNA, guiding it into the plant nucleus.
D) VirE2 is a single-stranded DNA-binding protein that coats the T-DNA, protecting it from degradation during transfer to the plant cell.
E) VirD1 functions as a helicase, unwinding double-stranded DNA to facilitate the entry of T-DNA into the bacterial replication machinery.

1
B,C,D
2
A,C,D
3
A,B,C
4
A,B,C,D,E
5
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