In the maturation of precursor tRNA (pre-tRNA) into functional tRNA within a eukaryotic cell, several modifications and processing steps are necessary. Which of the following steps correctly describes part of this maturation process?
1
Addition of an adenine ribonucleotide at the 5' end of the pre-tRNA to promote stability against nucleases.
2
Introns within the pre-tRNA sequence are spliced out by ribosomes to ensure proper folding and function of the tRNA.
3
The CCA sequence is added to the 3' end of the pre-tRNA if not encoded by the gene, providing the site for amino acid attachment.
4
The anticodon loop is enzymatically removed and replaced with a universally conserved sequence to enhance codon recognition efficiency.
5
Question Not Attempted