You are preparing 100 ml of a solution containing:
100 mM Tris‐HCl (pH 7.5);
5 mM MgCl ;
1 mM DTT.
If the stock solutions provided are: 1 M Tris‐HCl (pH 7.5); 100 mM MgCl ; 50 mM DTT, the amount of each component would be:
1
1M Tris HCl (pH 7.5) : 20 ml ; 100 mM MgCl2 : 2.5 ml ; 50 mM DTT : 5 ml
2
1M Tris HCl (pH 7.5) : 10 ml ; 100 mM MgCl2 : 5.0 ml ; 50 mM DTT : 2 ml
3
1M Tris HCl (pH 7.5) : 05 ml ; 100 mM MgCl2 : 10 ml ; 50 mM DTT : 2.5 ml
4
1M Tris HCl (pH 7.5) : 10 ml ; 100 mM MgCl2 : 7.5 ml ; 50 mM DTT : 10 ml
5
Question Not Attempted