Which one of the following statements regarding amphibian development is correct?

1
The Nieuwkoop centre is formed on the dorsal side of the embryo due to accumulation of β-catenin which helps activate the siamois and twin genes
2
The ectodermal cells form neural tissues in the presence of BMP molecules
3
Brain formation requires the activation of both Wnt and BMP pathway.
4
There is a gradient of Nodal-related protein across the endoderm with low concentration on the dorsal side of the embryo
5
Question Not Attempted

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