The steady state level of a plant metabolite 'M' is determined by the complex interplay of its biosynthesis, catabolism and transport processes from the source to the sink organ. A researcher tested following molecular and genetic strategies for engineering the metabolite 'M' in the native host plant.
A. Increasing catalytic efficiency of its rate-limiting biosynthetic enzyme in the source organ.
B. Increasing catalytic efficiency of the catabolic enzymes in the source organ.
C. Generating knock-out of the transporter protein in the source organ.
D. Repression of the catabolic enzymes in the sink organ.
Which of the above-mentioned strategies will provide a higher accumulation of the target metabolite 'M' in the sink organ?
1
A and B
2
B and C
3
C and D
4
A and D