Consider the following statements about enzymes used in recombinant DNA technology:
A. Restriction endonucleases cut DNA at specific sequences, creating either blunt or sticky ends.
B. DNA polymerase I from Escherichia coli is used to synthesize cDNA from mRNA.
C. Terminal deoxynucleotidyl transferase (TdT) adds nucleotides to the 3' ends of DNA without the need for a template.
D. RNase H specifically degrades the RNA strand of RNA-DNA hybrids.
Which combination of statements is correct?
1
A and C
2
B and D
3
A, C, and D
4
A and D