An enzyme has a Km of 1 mM. Addition of different inhibitors changes Km and/or Vmax given as \(\rm K_m^{app}\) and \(\rm V_{max}^{app}\) (app for apparent), respectively. Which one of the following inhibitors will result in the lowest rate of enzyme-catalyzed reaction?
1
An inhibitor with \(\rm K_m^{app} = 5 K_m\)
2
An inhibitor with \(\rm V_{max}^{app} = \frac{V_{max}}{5}\)
3
An inhibitor with \(\rm K_m^{app} = 3 K_m \,\,and\,\, V^{app}_{max} = \frac{V_{max}}{2}\)
4
An inhibitor with \(\rm K_m^{app} = 2 K_m \,\,and\,\, V^{app}_{max} = \frac{V_{max}}{3}\)