In vivo interaction between FLAG-tagged protein P and chaperone H was examined by co-immunoprecipitation (co-IP). Co-chaperone A was included and the co-IP was performed in the presence or absence of cycloheximide (CHX). The results of the co-IP experiments are shown below.
Which of the following interpretations from these experiments is INCORRECT?
1
Protein P interacts with chaperone H and co-chaperone A.
2
Chaperone H interacts only with the newly synthesized protein P.
3
Interaction between co-chaperone A and protein P is independent of chaperone H.
4
Interaction between chaperone H and protein P is independent of cochaperone A .