A DNA sequence is given below:
5' - ATGACGATGACGAGACGATGCAGATGATAGCAGTAGCGAATGAC - 3'
The following primers were designed to amplify the above sequence:
A. 5' - TACTGCT - 3'
B. 5' - CAGTAAG - 3'
C. 5' - ATGACGA - 3'
D. 5' - GTCATTC - 3'
E. 5' - TCGTCAT - 3'
F. 5' - GAATGAC - 3'
If we negate the effects of primer length, Tm, %GC and other factors, which one of the following options represents a combination of primers that could amplify the above DNA sequence?
1
A and C
2
B and D
3
E and F
4
C and D