In order to investigate the involvement of the following proteins in the mismatch repair mechanism (MMR), an in vitro reconstitution experiment was performed. A 5'-nicked circular DNA substrate having a C : C mismatch at the Pstl site was incubated with different combination of proteins (as shown below), where upon the repair of C : C mismatch, the Pstl site will be regenerated. Following the incubation, the resulting DNA were digested with Pstl and Scal restriction endonucleases, and the products were electrophoresed in 0.8% agarose gel.
Based on the results obtained identify the INCORRECT statement.
1
Msh2-Msh6 complex is required for the repair of the C:C mismatched DNA.
2
Polδ and Msh2-Msh6 complex are necessary for the repair of the C:C mismatched DNA.
3
Polδ and Msh2-Msh6 complex are sufficient for the repair of the C:C mismatched DNA.
4
Exol and Rad27 are redundant to each other for the repair of the C:C mismatched DNA.