A cancer cell line obtained from a rat glioma tumour was stained with the nuclear dye Hoechst 33342 and sorted using FACS. About 0.4% of the population stained lightly (LSP), distinct from the densely stained population of cells (DSP). Equal number of cells from these two populations were subcutaneously implanted into a suitable animal model to develop tumours. Following statements are made from this experiment:
A. The LSP cells will give rise to tumours.
B. The DSP cells will give rise to tumours.
C. The LSP cells can give rise to LSP and DSP cells.
D. The DSP cells can give rise to LSP and DSP cells.
Which one of the following options represents the combination of all correct statements?
1
A and D
2
A and C
3
B and C
4
B and D