In a muscle cell under conditions of strenuous exercise, the concentration of ATP, ADP, and Pi are measured to be 3.0 mM, 1.5 mM, and 5.0 mM, respectively. The standard free energy change (ΔG°) for ATP hydrolysis at pH 7.0 and 37°C is -30.5 kJ/mol. Given this data, what is the actual free energy change (ΔG) for ATP hydrolysis under these conditions?
1
-45.5 kJ/mol
2
28.1 kJ/mol
3
-65.5 kJ/mol
4
-30.5 kJ/mol