The following experimental manipulations were carried out with Xenopus embryo.

Manipulation X: Exposure to ultra violet radiation leading to the failure of cortical rotation.

Manipulation Y: Gastrulae treated with lithium chloride, an agonist of canonical Wnt signaling.

The following statements were made with respect to the above manipulations and genes involved in setting up dorso-ventral polarity in amphibians.

A. The phenotype obtained due to manipulation X can be rescued by injection of noggin in 1-cell embryo

B. Chordin mRNA will be enriched in embryos of manipulation X as compared to those of manipulation Y

C. Injection of cDNA for chordin into ventral blastomeres leads to the induction of a secondary axis.

D. Experimentally depleting β-catenin transcripts in 1-cell embryo by antisense oligonucleotides leads to phenotype similar to that obtained from manipulation X.

Which one of the following options represents all correct statements?

1
A, C and D
2
B, C and D
3
A and B only
4
B and C only

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